Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: Primer sequences, amplicon size, and gene accession number.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Amplification, Sequencing

Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: EGFL7 gene expression levels in chorionic villous explants immediately after dissection. ( A ): Representative phase-contrast images of villous explant samples from control and PE placenta. ( B ): qRT-PCR analysis demonstrating reduced expression of EGFL7 in PE villous explant samples compared to the healthy controls. Scale bar in panel images = 250 μM. Statistical analysis was performed using Mann–Whitney test (** p = 0.0044).

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Expressing, Dissection, Control, Quantitative RT-PCR, MANN-WHITNEY

Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: Effect of pravastatin administration to ex vivo chorionic villous explant cultures on EGFL7 expression. ( A ): Representative phase-contrast images of villous explants from healthy control and PE placenta after 24 h of culture in the presence or absence of 10 μM pravastatin (PRA). ( B ): qRT-PCR analysis showing that PRA treatment did not affect EGFL7 expression in both healthy control and PE villous cultures. Scale bar in panel images = 250 μM. Statistical analysis was performed using Student’s t test. − and + PRA: without or with 10 μM pravastatin.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Ex Vivo, Expressing, Control, Quantitative RT-PCR

Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: Identification of high-responder and low-responder PE pregnancies following treatment of chorionic villous explant cultures with 10 μM pravastatin (PRA) for 24 h. ( A , B ): qRT-PCR analysis demonstrating increased expression of EGFL7 in high-responder PE villous explant cultures following PRA treatment ( A ) and no changes in low-responder PE villous cultures ( B ) when compared to villi cultured without PRA. ( C ): Western blot analysis of villous explant cultures with or without PRA, indicating increased expression of EGFL7 in high-responder PE villous cultures. ( D ): Quantification of Western blot analysis ( n = 3). Statistical analysis was performed using Student’s t test (* p = 0.0467 for qRT-PCR; * p = 0.028 for western blot). − and + PRA: without or with 10 μM pravastatin.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Quantitative RT-PCR, Expressing, Cell Culture, Western Blot

Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: Maternal socio-demographic characteristics and biochemical findings of women with pre-eclampsia according to EGFL7 expression in response to pravastatin treatment.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Expressing

Journal: Biomedicines

Article Title: Effect of Pravastatin on Placental Expression of Epidermal Growth Factor-like Domain 7 in Early-Onset Pre-Eclampsia: A New Potential Mechanism of Action

doi: 10.3390/biomedicines12081929

Figure Lengend Snippet: Perinatal and neonatal outcome of women with pre-eclampsia according to EGFL7 expression in response to pravastatin treatment.

Article Snippet: Membranes were then incubated overnight at 4 °C with rabbit anti-EGFL7 (Abcam, Cambridge, UK, cat. ab256451, 1:1000) or mouse anti-GAPDH (clone 6C5, Santa Cruz, CA, USA, cat. sc-32233, 1:2000), all diluted in TBS/T with 5% ( w / v ) bovine serum albumin (BSA).

Techniques: Expressing

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: Primer sequences used in PCR.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques:

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: TMP promotes angiogenesis, nerve regeneration and nerve defect repair in rats with SCI. A: BBB score to test the motor behavior of rats; B–C: HE staining and TUNEL staining to assess the lesions of rat spinal cord; D: Western blot to detect CD31 expression to assess angiogenesis; E–G: ELISA to detect inflammatory factors; H–I: RT-qPCR or Western blot to detect miR-497-5p and EGFL7 expression; the values are expressed by the mean standard deviation. * P < 0.05 vs. Sham; # P < 0.05 vs. Model.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Staining, TUNEL Assay, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: MiR-497-5p inhibits EGFL7 expression. A: Bioinformatics website to predict that miR-497-5p and EGFL7 have targeted binding sites; B: Dual-luciferase reporter gene detection to confirm the targeting relationship between miR-497-5p and EGFL7; C –D: RT-qPCR and Western blot to detect EGFL7 expression; the values were expressed by the mean standard deviation. * P < 0.05 vs. TMP + antagomir NC; # P < 0.05 vs. TMP + agomir NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Expressing, Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: EGFL7 upregulation promotes TMP-mediated protection on SCI rats. A: RT-qPCR or Western blot to detect EGFL7 expression; B: BBB score to test the motor behavior of rats; C –D: HE staining and TUNEL staining to assess the lesions of the rat spinal cord; E: Western blot to detect CD31 expression to assess angiogenesis; F–H: ELISA detects the levels of inflammatory factors; the values are expressed by the mean standard deviation. * P < 0.05 vs. TMP + oe-NC; # P < 0.05 vs. TMP + sh-NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Heliyon

Article Title: Tetramethylpyrazine promotes angiogenesis and nerve regeneration and nerve defect repair in rats with spinal cord injury

doi: 10.1016/j.heliyon.2023.e21549

Figure Lengend Snippet: EGFL7 inhibition resists miR-497-5p silencing-induced protection against SCI. A: RT-qPCR or Western blot to detect EGFL7 expression; B: BBB score to test the motor behavior of rats; C –D: HE staining and TUNEL staining to assess the lesions of the rat spinal cord; E: Western blot to detect CD31 expression to assess angiogenesis; F–H: ELISA detects the levels of inflammatory factors; the values are expressed by the mean standard deviation. * P < 0.05 vs. TMP + miR-497-5p antagomir + sh-NC.

Article Snippet: After being separated by 12 % SDS-PAGE, the samples were loaded onto polyvinylidene fluoride membranes (Bio-Rad, USA) which were then counteracted with primary antibodies EGFL7 (19291-1-AP, 1:500, Proteintech), CD31 (ab28364, 1:500, Abcam), and GAPDH (2118, 1:1000, CST) and co-incubated with the secondary antibody (ab6734, 1:3000, Abcam).

Techniques: Inhibition, Quantitative RT-PCR, Western Blot, Expressing, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation